The W. M. Keck Three-Dimensional Fusion Microscope


Josef Kerimo, Ph.D.
Keck Facility Manager, CenSSIS
Northeastern University
302 Stearns
360 Huntington Avenue
Boston, MA 02115

phone: 617.373.2589
fax: 617.373.7783

kerimo@coe.neu.edu

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The Keck Microscope

Keck 3D Fusion Microscope is housed in the CenSSIS Laboratory. Along with other equipment there, it provides a testbed for new imaging ideas in collaboration between biologists and engineers. BioBED is a Northeastern University shared resource between Electrical and Computer Engineering and Biology. The microscope allows us to image samples with at least 5 different modalities (DIC, CRM, LSCM, TPLSM, and QTM) on the same stage.

This is the only microscope of its type in the world and we expect very large amounts of productive and elegant data to be produced, in addition to what is already published. Below are explanations of the specific modalities available and information on how to use the 3D fusion microscope.

Additional Equipment

An anti-vibration, air suspension table, which allows for high-resolution long-term imaging with minimal artifact, supports all of our equipment. We have a Prior motorized X-Y stage and a piezosystem jena motorized Z stage that allows for XYZT imaging. There is also a Bioptech Delta-T live cell chamber that maintains a range of temperature and atmospheric conditions for ultra-long term imaging experiments. We are in the process of developing a detent spindle stage that will allow for X-axis rotation of samples during XYZT imaging.