The W. M. Keck Three-Dimensional Fusion Microscope


Josef Kerimo, Ph.D.
Keck Facility Manager, CenSSIS
Northeastern University
302 Stearns
360 Huntington Avenue
Boston, MA 02115

phone: 617.373.2589
fax: 617.373.7783

kerimo@coe.neu.edu

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Sample Images

Mouse Embyonic Development

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Successive images are taken using DIC (grayscale) and quadrature microscopy. Deepening intensity (of the blue) in the quadrature indicates increased density (multiple cells)






Underdrawing Analysis with Boston Museum of Fine Arts

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A line is drawn on a block of wood. Part of the line is covered by paint. The purpose of this project is to be able to image the "underdrawing," or the portion of the line under the paint. In the top-left confocal reflectance image, moving from the bottom up, you can see where the paint hides the line. Top row of images collected using 488nm laser line. The successive images follow the line under thicker coats of paint (notice how the line all but disappears). Using the 780nm line (bottom row), the line is visible through the layers of paint. Images are registered.

















DIC image (upper) of whole mount Drosophila brain. Currently, we are looking at tyrosine hydroxylase localization using confocal fluorescent microscopy (lower).








Epifluorescent Overlay of Bovine Pulmonary Artery Endothelial Cells



















Molecular Probes prepared microscope slide containing multi-labeled bovine pulmonary artery endothelial cells (BPAEC). Texas Red-X phalloidin is labeling the F-actin, anti-bovine-tubulin mouse monoclonal 236-10501 in conjunction with BODIPY FL goat anti-mouse IgG antibody is labeling microtubules, and DAPI is labeling the nucleus.





Multimodal Image of Mouse Embryo





















All images are pseudocolored. Top left image is Differential Interference Contrast (DIC). Top right is Confocal Reflectance (CRM). Bottom Left is Two-Photon Fluorescence Microscopy (TPLSM). Bottom right is Optical Quadrature Microscopy (OQM). Registered images are of individual slices taken from a stack. Cells are stained with DAPI (for 2P.